[Effect of botulinum toxin type A on the expression of substance P, calcitionin gene-related peptide, transforming growth factor beta-1 and alpha smooth muscle actin A in wound healing in rats] Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery [Zhonghua Zheng Xing Wai Ke Za Zhi] Journal article | | Title | [Effect of botulinum toxin type A on the expression of substance P, calcitionin gene-related peptide, transforming growth factor beta-1 and alpha smooth muscle actin A in wound healing in rats] | | Author(s) | Wang L, Tai NZ, Fan ZH | | Institution | Plastic and Cosmetic Department, Renji Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China. | | Source | Zhonghua Zheng Xing Wai Ke Za Zhi 2009 Jan; 25(1):50-3. | | Abstract | OBJECTIVE: To investigate the effect of botulinum toxin type A on the expression of substance P (SP), calcitionin gene-related peptide (CGRP), transforming growth factor beta-1 (TGF-beta1) and alpha smooth muscle actin A (alpha-SMA) in wound healing.
METHODS: 60 rats were randomly divided into group C (control) group L (low-dose) and group H (high-dose), with 20 rats in each group. The wound-healing model was established by excision of four full-thickness skin (1 cm x 1 cm, around the injection site) on the back of all SD rats on the 7th day after BTA injection. The wound size was measured and the expression of SP, CGRP, TGF-beta1 and alpha-SMA in wound granulation tissue was assayed by immunohistochemical staining and computerized image analysis before operation, and 3 days, 7 days and 14 days after operation.
RESULTS: All the wounds healed 14 days after operation. The wound size in L and H group was not significantly different with that in C group on the 3rd day and 7th day after operation. The positive immuno-staining of SP, CGRP, TGF-beta1 and alpha-SMA in group L and H was significantly weaker than those in C group. Meanwhile, the positive immuno-staining of all above substances in H group was weaker than those in L group significantly.
CONCLUSIONS: Botulinum toxin type A can decrease the expression of SP, CGRP, TGF-beta1, and alpha-SMA in wound healing in a dose-dependent manner with no effect on the healing time. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19408727 |
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